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CRISPR-Cas9 (mander.xyz)
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[-] Contramuffin@lemmy.world 35 points 2 days ago

Explanation: CRISPR-Cas9 is most commonly known to be a gene editor, but since its initial discovery, people have found that with some minor tweaks, CRISPR can be made to do a bunch of other things, things that biologists could have only dreamed of previously.

If you introduce a mutation that deactivates the Cas9 protein, the entire CRISPR complex still binds to DNA. Then, you can essentially glue whatever you want onto this modified Cas9 to produce a bunch of different effects:

  • deactivated Cas9, no additional modifications: blocks other proteins from touching the DNA, which suppresses a gene without destroying it
  • glue on some transcriptional activators: makes the cell express a gene
  • glue on some fluorescent proteins: makes your DNA glow, lets you see where it is
[-] ChillCapybara@discuss.tchncs.de 12 points 2 days ago

Well done! I zoomed in and felt dumb. But thanks to your succinct explanation, I now have a sweet Dunning-Kruger high

this post was submitted on 02 Jul 2025
233 points (98.7% liked)

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