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submitted 6 days ago by fossilesque@mander.xyz to c/science_memes@mander.xyz
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[-] phdepressed@sh.itjust.works 24 points 6 days ago

No you add DMSO (5-10%) and freeze slowly. Using a Mr frosty or similar. Otherwise a few hours at -20, then -80, before the LN2.

Just chucking in LN2 is going to have terrible recovery. That might have been done with HeLa way back when but certainly isn't standard anymore.

[-] Beryl@jlai.lu 9 points 6 days ago* (last edited 6 days ago)

I mean obviously you'd use DMSO or glycerol, I just didn't want to get too technical. That being said I've always snap-frozen bacteria with LN2 and it worked just fine. Now for eucaryotic cells, sure, you'd want to go slow.

Edit : ok now re-reading the meme I understand why you thought I was talking about eucaryotic cells. My bad !

this post was submitted on 06 Aug 2025
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