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CRISPR-Cas9 (mander.xyz)
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[-] Contramuffin@lemmy.world 38 points 2 weeks ago

Explanation: CRISPR-Cas9 is most commonly known to be a gene editor, but since its initial discovery, people have found that with some minor tweaks, CRISPR can be made to do a bunch of other things, things that biologists could have only dreamed of previously.

If you introduce a mutation that deactivates the Cas9 protein, the entire CRISPR complex still binds to DNA. Then, you can essentially glue whatever you want onto this modified Cas9 to produce a bunch of different effects:

  • deactivated Cas9, no additional modifications: blocks other proteins from touching the DNA, which suppresses a gene without destroying it
  • glue on some transcriptional activators: makes the cell express a gene
  • glue on some fluorescent proteins: makes your DNA glow, lets you see where it is
[-] ChillCapybara@discuss.tchncs.de 13 points 2 weeks ago

Well done! I zoomed in and felt dumb. But thanks to your succinct explanation, I now have a sweet Dunning-Kruger high

[-] DeltaWingDragon@sh.itjust.works 22 points 2 weeks ago

Genetic engineering has never been this cute

[-] riskable@programming.dev 16 points 2 weeks ago

I dunno. For hundreds of thousands of years we've been selectively breeding puppies.

Puppies

[-] general_kitten@sopuli.xyz 11 points 2 weeks ago

In a way one could argue genetic engineering is one of the oldest sciences ever. And it started with dogs, or maybe agriculture, depends on what one's definition of science is.

[-] emergencyfood@sh.itjust.works 2 points 2 weeks ago

Whatever we did to pugs is definitely not cute. Or okay.

[-] BootyEnthusiast@lemmy.dbzer0.com 6 points 2 weeks ago

I want to >!fuck!< CRISPRi

[-] DeltaWingDragon@sh.itjust.works 8 points 2 weeks ago
[-] riskable@programming.dev 11 points 2 weeks ago* (last edited 2 weeks ago)

Your ~~spoiler~~ transcription repressor failed

FTFY ๐Ÿ‘

[-] Little8Lost@lemmy.world 4 points 2 weeks ago

Sadly there are two spoiler types i know of :/
for me the spoiler works because i use the "second one?"

[-] call_me_xale@lemmy.zip 4 points 2 weeks ago
[-] general_kitten@sopuli.xyz 8 points 2 weeks ago

"...that in terms of male human and female CRISPR-Cas9 variant reproduction, CRISPRi is the most compatible CRISPR-Cas9 variant for humans? Not only are they in the genetic perturbation group which is mostly comprised of various methods, CRISPRi are an average of..."

[-] xx3rawr@sh.itjust.works 1 points 2 weeks ago

Can't help it, it's most compatible

[-] ZILtoid1991@lemmy.world 1 points 2 weeks ago
[-] joenforcer@midwest.social -1 points 2 weeks ago

Y'all need Jesus. And an assigned FBI agent.

[-] ZILtoid1991@lemmy.world 3 points 2 weeks ago

It's a drawing! It's fiction! And if you come with the "normalizing" argument, then your "Y'all need Jesus" is normalizing the christofascism that will destroy this world.

[-] joenforcer@midwest.social 1 points 2 weeks ago* (last edited 2 weeks ago)

Y'all need therapy. Mostly for taking a joke waaaaaaay too seriously.

this post was submitted on 02 Jul 2025
247 points (98.4% liked)

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